Investigating Apoptotic Induction of Persian Species of Two Medicinal Mushrooms as New Herbal Medicines in Human Renal Cancer Cells Induced by A-498 Cell Line: A Preclinical Study
نویسندگان
1 Department of Urology, Mellannie Nutrition Research Institute, Manchester, UK
2 Department of Clinical Medicine, Golestan University of Medical Sciences, Gorgan, Iran
3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran
4 Department of Clinical Sciences, Faculty of Veterinary Medicine, Babol Islamic Azad University, Babol, Iran
5 Regenerative Medicine and Cell Therapy Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6 Regenerative Medicine and Cell Therapy Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7 Regenerative Medicine and Cell Therapy Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
8 Department of Clinical Sciences, Faculty of Veterinary Medicine, Babol Islamic Azad University, Babol, Iran
9 Eye Center of Xiangya Hospital, National Clinical Key Specialty of Ophthalmology, Hunan Key Laboratory of Ophthalmology, Central South University, Hunan, China
10 Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University of Kazerun Branch, Kazerun, Iran
11 Department of Medical Biotechnology, Faculty of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran
12 Department of Medical Biotechnology, Torbat Heydarieh University of Medical Sciences, Torbat Heydarieh, Iran
13 Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
14 Department of Developmental Biology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
doi
10.22034/tru.2025.509749.1230چکیده
Introduction: Renal Cell Carcinoma (RCC) accounts for approximately 85% of kidney cancer cases worldwide. While standard treatments such as surgery and targeted therapies are commonly used, they often exhibit limited efficacy, leading to drug resistance and severe side effects. Given these challenges, natural compounds, particularly medicinal fungi, have gained interest as potential alternative therapies. This study investigates the anticancer effects of Ganoderma lucidum and Trametes versicolor on RCC by assessing their ability to induce apoptosis and inhibit tumor growth in a murine model.Methods: RCC was induced in C57BL/6 nude mice via subcutaneous injection of A-498 renal cancer cells. Mice were divided into three groups: untreated control, Ganoderma-treated, and Trametes-treated groups. Extracts were administered via oral gavage for 28 days. Tumor volume and weight were recorded, and apoptosis was evaluated using the TUNEL assay. Data were analyzed using ANOVA with GraphPad Prism.Results: Mice treated with Ganoderma lucidum and Trametes versicolor exhibited significantly lower tumor volume and tumor weight compared to the control group (P-value< 0.0001). The TUNEL assay confirmed a higher percentage of apoptotic cells in treated groups (P-value< 0.0001), indicating increased tumor cell death.Conclusion: Our findings suggest that Ganoderma lucidum and Trametes versicolor possess significant anticancer properties against RCC by inducing apoptosis and reducing tumor burden. These results support further investigation into the mechanisms of action and potential clinical applications of medicinal fungi in RCC treatment.
