Comparison of Conventional Cigarette and Electronic Cigarettes on P53, PTEN, and VEGF Genes Expression in Rat Kidney
نویسندگان
1 Research Committee Member, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
2 Urology and Nephrology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3 Department of Biology, Medical Biotechnology Research Center, Yazd University, Yazd, Iran
4 Department of Genetics and Biotechnology, School of Biological Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
5 Department of Pathology, University of California, Los Angeles, USA
6 Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
7 Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
8 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
9 Department of Biology, Khatam University, Tehran, Iran
10 Department of Genetics, Medical Branch, Islamic Azad University, Tehran, Iran
11 Department of Pathology, AshianGanoTeb Biopharmaceutical Company, Golestan University of Medical Sciences, Gorgan, Iran
doi
10.22034/tru.2023.421288.1162چکیده
Introduction: Various genetic alterations and gene expression changes are involved in the pathogenesis of kidney tumours. The P53, PTEN, and VEGF gene expression pattern in rat kidney tissue exposed to conventional cigarettes and e-cigs was evaluated.Method: In the study, 60 Wistar rats were categorized into three groups (10): no smoke, c-cig smoke, and e-cig smoke. Three cigarette butts used daily with the suction device were exposed for 40 minutes. After four months, kidney tissue was removed, tissue RNA was extracted, cDNA was made, and changes in mRNA expression of genes were evaluated by real-time PCR. Moreover, kidney biopsies were assessed for histopathological changes.Result: E-cig smoke might contribute to kidney cancer more than C-cigarette through upregulating VEGF and downregulating PTEN and P53 (P-value >0.05). The effect of e-cigarettes in female rats was more significant than in male rats. The histopathological investigation revealed decreased glomerular space in e-cig male rats, increased tubular necrosis in e-cig, Interstitial infiltration in c-cig, and Hyperemia in c-cig in female rats were reported.Conclusion: Since E-cigarette smoke displayed overexpressed VEGF and down-expressed PTEN and P53 expression than C-cigarette, it has a higher potential for kidney carcinogenesis.
