Molecular characterization of recent Iranian infectious bronchitis virus isolates based on S2 protein gene
نویسندگان
1 Department of Microbiology and immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran - IRAN
2 Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, IRAN.
3 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IRAN.
4 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IRAN.
5 Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, IRAN.
6 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
doi
10.22059/ijvm.2016.59716چکیده
Background: Avian infectious bronchitis (IB), with avianinfectious bronchitis virus (IBV) as the causing agent, is a ubiquitous endemicdisease of the chicken with devastating effects on its industry. A viralmembrane surface protein called S notonly induces neutralizing antibodies but also plays an important role in virusbinding and entry to host cells. Technically, S1 protein gene sequencing alsohelps greatly in IBV genotyping. OBJECTIVES: The aim of this study wasto characterize Iranian IBV based on S2 gene. METHODS: After RT-PCRamplification, the S2 gene of nine Iranian IBV isolates were sequenced and thencompared with reference strains. RESULTS: The isolates were classifiedinto genotype I as Massachusetts like IB Vs, genotype VII which clustered intotwo branches, VIIa (IS-1494 like IB viruses), and VIIb, and was related to QX-like viruses and Genotype VIII as 793/B like IBVs. CONCLUSIONS: As faras we know, this is the first S2-based classification study on Iranian IBVisolates providing a firm experimental basis to correlate with genotypiccharacterization
