فهرست نشریات علمی دسترسی آزاد ایران

In recent times, there has been a lot of attention given to iron oxide nanoparticles (IONPs) because of the exceptional physicochemical characteristics, biocompatibility and multi-functional therapeutic properties of this product. In this paper, IONPs have been prepared in aqueous medium through the pulsed laser ablation of an iron target with 300, 400 and 500 mJ power and discussed by X-ray diffraction (XRD) and field emission scanning electron microscopy (FESEM). XRD analysis ensured that the product was highly pure iron oxide with a cubic spinel structure, whereas FESEM showed irregular and agglomerated nanoparticles with a size ranging between 46.71 and 142.6nm and a predominantly spheric to slightly polyhedric morphology, which are indicative of a polydisperse system. Through a crystal violet cytotoxicity assay, the anticancer property of IONPs was compared to the HepG2 cancer cells and RD normal cells. IONPs prepared at 500 mJ had the best growth inhibition in both cell lines and this was due to the higher concentration of nanoparticle, small size and high surface area. Interestingly, it was observed that the cytotoxicity profile caused the selective induction of the apoptosis of cancer cells with relatively reduced impact on the normal cells, thus showing some selectivity. After 5 minutes of radiation of 532 nm laser diode, IONPs produced localized photothermal heating and reactive oxygen species (ROS), resulting in the oxidative stress, mitochondrial damage, and apoptosis in tumor cells. Photodynamic effect coupled with photothermal effect, combined with intrinsic magnetic responsiveness of IONPs, offers a flexible system of spatially controlled and minimally invasive cancer therapy. These results indicate that laser ablation is a clean, controllable route to fabricate magnetic IONPs that would have potential applications in multimodal anticancer therapies.