فهرست نشریات علمی دسترسی آزاد ایران

Objective(s): Zinc oxide nanoparticles (ZnO NPs) can induce toxicity in living organisms. Origanum vulgare (O. vulgare), is a medicinal plant which has antioxidant components and traditionally used to treat different health problems in Iran. In this study, we assessed the potential protective effect of O. vulgare extract against ZnO NPs cytotoxicity in L-929 cells. Methods: In the first group, different concentrations of ZnO NPs, and in the second group, one dose of O. vulgare extract plus different concentrations of ZnO NPs were exposed to L-929 cells for 24 hours. After treatment, cytotoxicity of different concentrations of ZnO NPs on the cells and protective effect of O. vulgare extract against cytotoxicity of ZnO NPs were examined microscopically. Then, the neutral red uptake and MTT assays were performed to determine cell viability.Results: Higher concentrations of ZnO NPs showed significant morphological abnormalities on the cells. O. vulgare extract had no cytotoxic effect on L-929 cells. It could protect and reduce cytotoxicity of higher concentrations of ZnO NPs in cells. The viability of L-929 cells after 24 hours of exposure to ZnO NPs varied depending on the nanoparticles concentration. The results of MTT and NRU assays showed that different concentrations of ZnO NPs induced cell mortality and O. vulgare extract could significantly decline cell mortality induced by ZnO NPs.Conclusions: It can be concluded that O. vulgare extract has potent protective effect against the cytotoxicity induced by ZnO NPs on L-929 cells.