فهرست نشریات علمی دسترسی آزاد ایران

Trichomonas vaginalis is a protozoan parasite responsible for trichomoniasis, a prevalent sexually transmitted infection. Metronidazole is the standard treatment; however, concerns regarding side effects and emerging resistance necessitate the exploration of alternative therapies. This study aimed to evaluate the antiparasitic activity of silver nanoparticles coated with apigenin (Api@AgNPs) against T. vaginalis trophozoites in vitro. The nanoparticles were synthesized via a green synthesis method and characterized using UV-Vis spectroscopy and transmission electron microscopy, confirming an average size of 38.3 nm with a quasi-spherical morphology. Trophozoites were exposed to varying concentrations of Api@AgNPs and metronidazole for 24 and 48 hours. Viability was assessed using Trypan blue staining, and CC₅₀ values were determined by probit regression. Api@AgNPs exhibited CC₅₀ values of 111.211 µg/mL and 81.331 µg/mL at 24 and 48 hours, respectively, compared to 107.09 µg/mL and 76.542 µg/mL for metronidazole. These findings suggest that Api@AgNPs possess promising anti-trichomonal activity and may serve as a potential therapeutic agent alone or in combination with metronidazole, pending further in vivo validation.