فهرست نشریات علمی دسترسی آزاد ایران

Objective(s): MgSO4, a calcium (Ca) channel blocker, can disrupt ionic homeostasis. However, its effect on the synergy of nanomaterials in static magnetic fields (SMF) with therapeutic use has not been investigated in an in vivo model. We used the blocker alone and with CuO nanoparticles (NPs) to investigate the synergy of SMF and NPs in living P. caudatum.Methods: The experimental model was grown in straw and purified after serial cultivation. The pure environment was then divided into two groups. One group was exposed to SMF (0.061 mT) for 72 hours and the second group was exposed to natural, geostatic laboratory conditions. A sample of each group (0.1 mL) was treated with MgSO4 or/and CuO NPs (1, 3, 9 μg/μL) under a fixed objective lens (4x) for 25 times in 30 seconds with 5-second time interval. The control group received only 1 μL of distilled water. Sigmoid and avoidance movements of paramecia were counted. Trichocyst, macronucleus, pellicle, Ca channel and nitric oxide synthase (NOS) activation were studied. All data were analysed using ANOVA (α = 0.05).Results: No changes in Ca channel density or NOS activation were observed due to exposure of paramecia to MgSO4 alone. However, the movement of paramecia using MgSO4 with high doses of CuO NPs under SMF was reduced, and the pellicular and macronuclear units were destroyed, indicating increased toxicity of CuO NPs. Conclusions: MgSO4 can affect the diamagnetic state of CuO NPs under SMF, thereby intensifying the non-protective effects of nanomaterials.