Investigation of Effective Parameters on Aptamer-Based Electrochemical Sensor for Rapid Detection of Tetracycline Residues in Chicken Ham
نویسندگان
1 Department of Food Science and Technology, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 Department of Food Science and Technology, North Tehran Branch, Islamic Azad University, Tehran, Iran
3 Venom and Biotherapeutics Molecules Lab, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
doi
10.22034/nmrj.2024.04.010چکیده
This study investigates the impact of key parameters, including aptamer concentration, aptamer incubation time, tetracycline (Tet) incubation time, pH, and temperature, on the peak current of an aptasensor designed for Tet detection. The aptasensor was developed by immobilizing a Tet aptamer onto gold nanoparticles (AuNPs) electrodeposited on an electrospun carbon nanofibers (CNFs). The integration of CNFs and AuNPs enhances the sensor's performance by increasing the surface area and improving conductivity. The results show that aptamer concentration, aptamer incubation time, incubation time of Tet, pH, and temperature affect the peak current of the aptasensor, demonstrating the importance as effective parameters in the preparation of biosensors.
