فهرست نشریات علمی دسترسی آزاد ایران

Objective(s): Nanofibrous scaffolds provide an environment similar to the body's extracellular matrix for cells, which affects cell morphology, adhesion, proliferation, and function.  The present study aimed to investigate the biocompatibility properties of electrospun polycaprolactone-gelatin (PCL/Gel) scaffold containing layered double hydroxide (LDH) nanoparticles on mouse L929 fibroblast cells. Methods: In this study, LDH nanoparticles were synthesized by co-precipitation method. A scaffold made of polycaprolactone and gelatin with 1% wt of optimized nanoclay was fabricated using electrospinning method. The synthesized nanoparticles were evaluated by XRD method and electron microscopy study. 2×104 L929 fibroblast cells were seeded on the scaffolds for 3 to 5 days. Then, different grades of ethanol were used to dehydrate the scaffolds. After drying the scaffolds, scanning electron microscopy was used to characterize and examine the fixed cells, and MTT test was used to evaluate the toxicity of the scaffolds.Results: The results of the present study showed that with the addition of LDH reduced the fiber diameter and increased the biocompatibility of the scaffold after culturing mouse L929 fibroblast cells. Also, scanning electron microscope images indicated better interaction and proliferation of L929 cells on PCL/Gel/LDH compared to the PCL/Gel group.Conclusions: Adding LDH to PCL/Gel nanofibers is effective on the adhesion and viability of cells.