Mesenchymal stem/stromal (MSCs)-derived exosome inhibits retinoblastoma Y-79 cell line proliferation and induces their apoptosis
نویسندگان
1 Department of Pathology, Imam Khomeini Hospital, Urmia University of Medical Sciences, Urmia, Iran
2 Isfahan Eye Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
3 KIMS Health Hospital, Oman
4 Master of Science Graduated from Shahid Beheshti Medical University
doi
10.22034/nmrj.2022.03.006چکیده
Aims: Based on the literature, mesenchymal stem/stromal cell derived exosome has dual role in tumor therapy or progress. We evaluated the possible anti-tumor effect of the MSCs-exosome on retinoblastoma Y-79 cell cells in vitro.Methods: To verify the efficient isolation of exosome, the MSCs-exosome structure and the expression of CD9 and CD81 was examined using transmission electron microscopy (TEM) and western blotting, respectively. Y-79 cell proliferation and apoptosis were evaluated using MTT assay and flow cytometry, respectively. The expression levels of Bcl-2 and microRNA-143 were estimated by the Real-time PCR.Results: MSCs-exosome inhibited Y-79 cells proliferation as shown by MTT assay results. Also, MSCs-exosome induced apoptosis in Y-79 cells within 72 hours of treatment. Real-time PCR results also exhibited that exosome therapy resulted in a decrease in Bcl-2 expression while up-regulating microRNA-143 expression.Conclusion: MSCs-derived exosome could deter retinoblastoma Y-79 cells tumorigenicity, thereby providing novel modality to manage retinoblastoma patients.
