Silver Nanoparticle-Induced Lymphocyte Activation in Zebrafish
نویسندگان
1 Laboratoire de Biochimie, Ecole Nationale de Médecine Vétérinaire, 2020 Sidi Thabet, Université de la Manouba, Tunisie
2 Laboratoire de Pharmacie et Toxicologie, Ecole Nationale de Médecine Vétérinaire, 2020 Sidi Thabet, Université de la Manouba, Tunisie
3 FAO Subregional Office for North Africa, rue du Lac Winnipeg, Les Berges du Lac 1, Tunis, Tunisia
4 Laboratoire d'Anatomie-Pathologique, Université de la Manouba, École Nationale de Médecine Vétérinaire de Sidi Thabet, 2020 Sidi Thabet, Tunisie
5 Laboratoire de Biochimie, Ecole Nationale de Médecine Vétérinaire, 2020 Sidi Thabet, Université de la Manouba, Tunisie
6 Département de Biologie BP.12, Université Badji Mokhtar-Annaba Faculté des Sciences, 23000 Annaba, Algérie
7 Centre de Recherche en Transplantation et Immunologie, UMR1064, Nantes, France
8 Laboratoire de Biochimie, Ecole Nationale de Médecine Vétérinaire, 2020 Sidi Thabet, Université de la Manouba, Tunisie
9 Laboratoire de Physiologie, Faculté des Sciences de Bizerte, Université de Carthage, Tunisie
doi
10.22034/nmrj.2022.02.009چکیده
Silver nanoparticles (Ag-NPs) are among the most widely used nanomaterials in the world. Nanotoxicological data on these additives remain unclear. The aim of this study is to evaluate the in vivo effects of Ag-NPs bioaccumulation on the hematological cells of adult zebrafish Danio rerio after three weeks of oral exposure. Four concentrations of Ag-NPs (65 nm nanosphere), are used: control group 1 (0.00 g/kg of food), group 2 (0.05 g/kg of food), group 3 (5 g/kg of food) and group 4 (50 g/kg of food). The experiment is conducted in triplicate. Cytological and histological results show, a significant increase in neutrophils associated with a significant decrease in lymphocytes in both group 3 and 4 compared to the control group (p<0,05 and p<0,005, respectively). This reflects an elevated neutrophil/lymphocyte ratio (NLR), suggesting a biased immune response associated with hyperplasia of the hematopoietic tissue and histopathological images of nephrological lesions, following medium to high dose of Ag-NPs intake. Besides, microscopic observation of cell morphology revealed lymphocyte activation only in groups 2 and 3 (p<0,005 and p<0,05, respectively). These results suggest that Ag-NPs induce a dose-dependent bioeffects on the immune system in zebrafish. This study reports that at low doses, Ag-NPs may activate lymphocytes but do not alter the NLR, thus demonstrating an immunostimulatory effect. The present investigation is the first study, as far as we know, reporting that Ag-NPs induce a dose-dependent immunomodulation in zebrafish.
