Excess iron ion reduction in a thalassemia model using silver nanoparticles modified by the tannin fraction of Myrtus communis extact
نویسندگان
1 Pharmaceutical Sciences Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
2 Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran
3 Pharmaceutical Sciences Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
4 Department of Biology, Sana Institute of Higher Education, Sari, Iran
5 Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Department of Medicinal Chemistry, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
6 Department of Basic Sciences, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran
7 Pharmaceutical Sciences Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
8 Department of Medical Entomology and Vector Control, School of Public Health and Health Sciences Research Center, Mazandaran of University of Medical Sciences, Sari, Iran
9 Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Department of Medicinal Chemistry, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
doi
10.22034/nmrj.2020.04.007چکیده
Objective(s): Nowadays, iron ions intoxication is the center of attention of interest in the management and treatment of thalassemia and different sorts of anemia associated with regular blood transfusions. Due to the major side effects of current drugs, they should be replaced with safer alternatives. Thus, in this study, functionalized hybrid silver nanoparticles, as an emerging perspective, were investigated for absorbing excess iron ions and their removal in an animal thalassemia model. Methods: The silver nanoparticles were green-synthesized using the Myrtus communis leaf methanolic extact (MC-AgNPs). The produced hybrid nano-Sorbents based on hydrolyzable tannin matrix loaded with silver nanoparticles were delivered for trying out in vivo iron chelation in thalassemia model mice in which iron-overload was imposed. MC-AgNPs and desferral were injected intraperitoneally four times a week for one month in mice with excess iron load. The total iron and Fe3+ content of serum was evaluated with the aid of plasma-atomic spectrometry microscopy and a Fe3+ ion measurement kit, respectively. Also, liver enzyme levels were evaluated by an auto-analyzer. Also, hepatic enzyme levels were appraised by using an auto-analyzer based the corresponding kits. Morphological transformations of the liver tissue were investigated by way of Prussian blue staining. Results: The mice treated with the MC-AgNPs demonstrated a significant reduction in serum iron content when compared with the iron-overload mice. MC-AgNPs revealed satisfying effectiveness to chelate excess iron in mice. Conclusions: This method could be considered as a competitive option for lowering the level of excess iron in vivo.
