Bio efficacy, and photocatalytic activity of the silver nanoparticles synthesized from Cryptolepis buchanani leaf extract
نویسندگان
1 Department of Biotechnology, Panskura Banamali College, P.O. – Panskura R.S., Purba Medinipur, West Bengal, PIN- 721152, India
2 Department of Microbiology, Vidyasagar University, Medinipur, West Bengal, PIN - 721102, India
3 Department of Biotechnology, Panskura Banamali College, P.O. – Panskura R.S., Purba Medinipur, West Bengal, PIN- 721152, India
4 Department of Biotechnology, Panskura Banamali College, P.O. – Panskura R.S., Purba Medinipur, West Bengal, PIN- 721152, India
5 Department of Biotechnology, Panskura Banamali College, P.O. – Panskura R.S., Purba Medinipur, West Bengal, PIN- 721152, India
6 Department of Biotechnology, Panskura Banamali College, P.O. – Panskura R.S., Purba Medinipur, West Bengal, PIN- 721152, India
doi
10.22034/nmrj.2020.04.009چکیده
The objective of the study is to synthesis silver nanoparticles from the leaf extract of Cryptolepis buchanani, a medically important plant,by green synthesis method. The synthesized nanoparticles have an average size of 17.05±5.27 nm, crystalline in nature with face cantered cubic structure, and positive surface charge. The nanoparticles are biologically active. It killed > 90 % of HeLa cells at 25 μg mL-1 concentration in-vitro cell cytotoxicity assay, with a LD50 value of 3.98 μg mL-1. The nanoparticles are less effective on MCF-7 and HEK-293 cell line, almost 70 % of MCF-7 populations were survived at highest concentration of 25 μg mL-1. In case of HEK-293 it killed almost 78 % of cells at the same concentration with a 9.45 μg mL-1 LD50 value. The synthesized nanoparticle lysed less than 2.5 % of red blood cells at 10 μg mL-1 concentration in-vitro. The nanoparticles degraded >90 % of methylene blue dye in presence of light in 8.5 h. These properties of the synthesized nanoparticles are unique, and make it promising for its future potential applications.
