Evaluation antibacterial activity of Biosynthesized Silver Nanoparticles by using extract of Euphorbia Pseudocactus Berger (Euphorbiaceae)
نویسندگان
1 Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
2 Social Security Organization,shahid beheshti hospital,shiraz,Iran
3 Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
4 Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
5 Department of Material Engineering, Shiraz University of Technology, Shiraz, Iran
6 Department of Basic Science, Payame Noor University, Iran
7 Department of Chemistry and Nanochemistry, Faculty of Sciences & Modern Technologies
8 Department of Medical Microbiology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
doi
10.22034/nmrj.2020.03.007چکیده
In the present study, silver nanoparticles (Ag NPs) were synthesized by using Euphorbia Pseudocactus Berger (Euphorbiaceae) extract, which played the main role in the formation and stability of nanoparticles. The physic-chemical property of biosynthesized nanoparticles were identified using X-ray diffraction (XRD), Ultraviolet–Visible spectroscopy (UV-Vis), Fourier-Transform Infrared spectroscopy (FT-IR) and Transmission Electron Microscopy (TEM) techniques. UV-Vis results illustrated that maximum plasma resonance absorption of Ag NPs are about 426 nm. Size distribution and spherical morphology was determined by TEM method. The XRD was confirmed face centered cubic (FCC) structure for synthesized nanoparticles.The molecular dynamics (MD) and monte carlo (MC) simulations were used to evaluate the nanoparticles .The antibacterial properties of biosynthesized Ag NPs were studied on E.coli (ATCC 25922), S. aureus (ATCC 2592), P. aeruginosa (ATCC27853) and E. faecalis (ATCC51299) by using micro dilution broth method. The minimum inhibitory concentration (MIC) results of synthesized Ag NPs on S. aureus and E. faecalis obtained 4 and 8 μg/mL and P. aeruginosa and E. coli obtained 16 and 4 μg/mL. So, synthesized nanoparticles can be utilized as an antibacterial agent in medical and industrial devices and tools.
