Phylogenetic study based on the phosphoprotein gene of Iranian Newcastle disease viruses (NDV) isolates, 2010 -2012
نویسندگان
1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, Iran
2 Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4 Department of Research & Production of Veterinary and Poultry Vaccines, Razi Vaccine and Serum Research Institute, Karaj, Iran
5 Department of Poultry Health and Diseases, Iranian Veterinary Organization, Tehran, Iran
6 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
7 Students' Scientific Association of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
8 Students' Scientific Association of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
doi
10.22059/ijvm.2014.51403چکیده
BACKGROUND: Newcastle disease virus (NDV) is the causativeagent of the Newcastle disease (ND), a highly contagiousdisease in birds that causes significant economic losses to thepoultry industry worldwide. ND is endemic in Iran and outbreaksare reported regularly in commercial poultry flocks and differentspecies of birds. OBJECTIVES:The current study was carried outto characterize NDV based on phosphorprotein (P) gene fromrecent outbreaks in Iran, 2010-2012. METHODS: The P genefragment of NDV isolates of five chickens, 1 ostrich, and 1Pigeon paramyxovirus-1 was obtained by RT-PCR and sequenced.RESULTS: Phylogenetic analysis of sequences revealed thatchicken and ostrich NDV isolates were closely related andplaced in the genotype VII and Pigeon Paramyxovirus-1 waslocated in the genotype V. CONCLUSIONS:This is the first reportof Phosphoprotein gene sequences of NDV strains isolated inIran. This study will help us to understand the epidemiology andmolecular characteristics of Newcastle disease virus in Iran.
