فهرست نشریات علمی دسترسی آزاد ایران

In this study, silver nanoparticles (AgNps) were successful synthesized using honey as antural, ecofriendly, cost effective and stabilizing agent. The formation of AgNPs was confirmed using several characterization techniques included (UV)visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), and energy dispersive X-ray spectroscopy (EDS). The results indicated that the synthesized AgNps were stable, solid and uniformly distributed, these finding suggest that honey can be effectively used as a green alternative for synthesis silvers nanoparticles. The antibacterial efficacy of the biosynthesized AgNPs was examined against Staphylococcus aureus and Pseudomonas aeruginosa utilizing the agar well diffusion method and minimum inhibitory concentration (MIC) assay. The findings demonstrated significant antibacterial efficacy against both bacterial types. The antibiofilm efficacy of AgNPs was assessed via the crystal violet microtiter plate assay, revealing a substantial decrease in biofilm development in a concentration dependent manner. The synergistic antibacterial and antibiofilm results demonstrate that honey-mediated AgNPs possess significant antimicrobial efficacy. The results indicate that biosynthesized AgNPs may be viable candidates for biomedical applications, especially in the prevention of biofilm-associated illnesses and the creation of antimicrobial coatings.