فهرست نشریات علمی دسترسی آزاد ایران

The manufacture of nanoparticles using plant extracts is an important and useful biological process compared to other manufacturing methods. The reason for this is that the biological or green nanotechnology method where there is no problem in maintaining and preserving plant cell cultures, so there is no fear of mutation in the preparation medium, as is the case with bacterial or fungal cultures. It is also easy to prepare and manufacture, usually in a few quick steps, and is inexpensive. The aim of this research was to find a safer, more environmentally friendly, inexpensive, and harmless method of synthesizing silver nanoparticles from Rosmarinusofficinalis extract and using them as a fungicide. The nanoparticles (silver nanoparticles) were Biosynthesized by using the aqueous extract of the rosemary plant and using it as a biological agent to reduce silver nitrate to nano-sized silver ions, by distilling the plant extract on the silver nitrate solution and observing the color change process, which indicates the formation of silver nanoparticles. Scientific tests confirming the green manufacturing process were conducted. Silver nanoparticles were then used as a fungicide in the laboratory for fungi that produce certain mycotoxins. Silver nanoparticles can be synthesized from R. officinalis leaf extract. The silver nanoparticles appeared to have a semi-pyramidal shape with a size of 63.65 nm when examined by Scanning Electron Microscopy, UV/Vis spectroscopy showed a peak at a wavelength of 345 nm, while X-ray diffraction showed four distinct diffraction peaks that matched the standard with which it was compared. The Atomic Force Microscopy examination showed that the size of the surface topography was 29.65 nanometers. Test results showed the effectiveness of three concentrations of silver (Ag) nanoparticles on certain pathogenic fungi that produce mycotoxins. The results of the experiment demonstrated the effectiveness of these nanoparticles. was very high in inhibiting the studied fungal species on PDA medium. It was observed that the percentage of inhibition increased with increasing concentrations, as the inhibition results at the concentration (ppm /L 100) of silver nanoparticles showed 100% complete inhibition with the two species Aspergillusnigerand Fusariumsolani, while 73.3% inhibition was observed for the same concentration with F. verticillioides. The study confirmed that the biosynthesis of nanoparticles through the use of plant extracts is a safe, fast, inexpensive and effective method. The study showed that nanoparticles prepared from rosemary extracts can be used as an antifungal agent against mycotoxins in the laboratory, with an inhibition rate of 100%.