فهرست نشریات علمی دسترسی آزاد ایران

The rapid expansion of green nanotechnology has driven renewed interest in environmentally safe approaches for producing biomedical nanomaterials. Among these, zinc oxide nanoparticles (ZnONPs) have attracted considerable attention due to their biocompatibility, chemical stability, and notable anticancer properties. Their nanoscale dimensions enhance cellular interactions and promote selective toxicity toward malignant cells, making them promising candidates for therapeutic development. In this study, ZnONPs were synthesized using an aqueous extract of Urtica pilulifera leaves as a natural reducing and stabilizing agent. Phytochemical screening confirmed the presence of flavonoids, phenolics, tannins, alkaloids, and terpenoids, all of which contributed to nanoparticle formation. The synthesized particles were characterized using FE-SEM to verify their optical, structural, and morphological features, UV–Vis spectroscopy, XRD, FTIR, and. Cytotoxic effects were evaluated against U87 glioblastoma cells using the MTT assay. Characterization revealed that the ZnONPs were spherical, crystalline, and coated with phytochemicals from the plant extract. Biological assays demonstrated a concentration-dependent reduction in U87 cell viability, accompanied by clear apoptotic indicators such as cell shrinkage and membrane disruption. The findings highlight the strong anticancer potential of green-synthesized ZnONPs, emphasizing their suitability as eco-friendly and effective agents for future applications in nanomedicine.