Green-Synthesized Silver Nanoparticles: Cytotoxic Effects on MDA-MB-231 and MCF-7 Cancer Cell Lines and Physicochemical Characterization
نویسندگان
1 Department of Physiology and Medical Physics, College of Medicine, Al-Nahrain University, Baghdad, Iraq
2 Department of Physiology and Medical Physics, College of Medicine, Al-Nahrain University, Baghdad, Iraq
3 Department of Physiology and Medical Physics, College of Medicine, Al-Nahrain University, Baghdad, Iraq
doi
10.22052/JNS.2026.03.027چکیده
The application of nanotechnology in cancer therapy has attracted significant attention in recent years. Among various nanomaterials, silver nanoparticles (AgNPs) have demonstrated promising potential due to their relatively low toxicity toward normal cells, cost-effectiveness, and potential ability to selectively target cancer cells. This study aimed to evaluate the inhibitory biological activity of silver nanoparticles against two human breast cancer cell lines, MCF-7 and MDA-MB-231. Silver nanoparticles were characterized using several analytical techniques including X-ray Diffraction (XRD), Ultraviolet-Visible Spectroscopy (UV-VIS), Energy Dispersion X-ray (EDX), Atomic Force Microscopy (AFM), and Filed Emission Scanning Electron Microscopy (FE-SEM). The characterization results confirmed the successful synthesis of quasi-spherical silver nanoparticles with particle sizes ranging from 30 to 40 nm. The cytotoxic effect of the synthesized AgNPs was investigated using the MTT assay on both MCF-7 and MDA-MB-231 cell lines at six different concentrations. The results indicated a concentration-dependent inhibitory effect of silver nanoparticles on both cancer cell lines, with a stronger cytotoxic activity observed against the MDA-MB-231 cell line. These findings highlight the potential of silver nanoparticles as promising anticancer agents against breast cancer cells and support the need for further investigations using more advanced biological models.
