Development of a New Ag Nanoparticles Decorated on Graphene Oxide (AgNPs@GO) as Colorimetric Sensor for Sensitive Cefazolin Determination in Complex Matrices
نویسندگان
1 Department of Internal Diseases, Endocrinology, Tashkent State Medical University, Tashkent, Uzbekistan
2 Department of Pediatrics, Faculty of Medicine, Samarkand State Medical University, Samarkand, Uzbekistan
3 Gulistan State University, Gulistan, Uzbekistan
4 Tashkent University of Information Technologies named after Muhammad al- Khwarizmi, Tashkent, Uzbekistan
5 Department of Neurology, Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
6 Department of Obstetrics and Gynecology, Tashkent State Medical University, Tashkent, Uzbekistan
7 Department of Neurology, Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
8 Department of Internal Diseases, Endocrinology, Tashkent State Medical University, Tashkent, Uzbekistan
9 Department of Biology, Gulistan State University, Gulistan, Uzbekistan
10 Department of Biology, Bukhara State University, Bukhara, Uzbekistan
11 Department of Pediatric Dentistry, Andijan State Medical Institute, Andijan, Uzbekistan
12 Department of Pediatric Dentistry, Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
13 Department of Neurology, Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
doi
10.22052/JNS.2026.03.032چکیده
Herein, we describe the development of a novel colorimetric sensor based on silver nanoparticles decoratively anchored onto a graphene oxide scaffold (AgNPs@GO) for the sensitive and selective determination of cefazolin in complex biological and environmental matrices. The nanocomposite was synthesized through an in-situ citrate-mediated reduction strategy, affording spherical silver nanoparticles with a mean diameter of 18.6 ± 4.2 nm uniformly dispersed across the graphene oxide surface. Comprehensive characterization employing FE-SEM, FT-IR, and XRD confirmed the successful immobilization of highly crystalline, phase-pure silver nanoparticles onto the exfoliated carbon support. The sensor operates on the principle of analyte-induced aggregation, transduced as a ratiometric change in the localized surface plasmon resonance absorption (A₆₅₀/A₄₀₀). Under optimized conditions (pH 7.0, 50 mM NaCl, 12 min incubation, 0.25 mg·mL⁻¹ AgNPs@GO), the probe exhibited a linear response toward cefazolin across the concentration range of 0.5 to 75.0 µM, with a limit of detection of 0.16 µM. The sensor demonstrated excellent selectivity against structurally analogous antibiotics and common coexisting species, with notable tolerance to ionic strength up to 100 mM NaCl. Practical applicability was validated through quantitative recovery of cefazolin from spiked human serum (96.0–98.9%), river water (98.0–99.6%), and wastewater effluent (94.0–97.8%), with results statistically equivalent to those obtained by high-performance liquid chromatography. This AgNPs@GO platform reconciles operational simplicity with robust analytical performance, presenting a compelling alternative to conventional instrumentation for antibiotic monitoring in resource-limited settings.
