فهرست نشریات علمی دسترسی آزاد ایران

Multidrug resistant (MDR) bacteria emergence is a major threat to the worldwide health care and novel antimicrobial agents have been developed. The eco friendly, cost effective and environmentally sustainable synthesis of zinc oxide nanoparticles (ZnO nanoparticles) using pomegranate peel extract (Punica granatum L.) as a bio reducing and stabilizing agent is revealed in this study. Ultraviolet-Visible Spectroscopy (UV-Vis), X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), and Fourier Transform Infrared Spectroscopy (FTIR) were utilized to examine the synthesized ZnO nanoparticles. UV-Vis analysis showed a characteristic absorption peak at 301 nm, and XRD analysis showed a hexagonal wurtzite crystalline structure with an average crystallite size of about 11 nm. TEM imaging showed ultra-fine, quasi spherical primary nanoparticles, mostly in the size range of 4 to 8 nm, belonging to the domain of quantum confinement. FTIR confirmed the presence of organic corona on nanoparticle surface as a result of the existence of pomegranate phytoconstituents such as polyphenols and flavonoids. The antimicrobial activity was performed on clinical multidrug resistant isolates of Staphylococcus aureus, Acinetobacter baumannii and Klebsiella pneumoniae by using agar well diffusion method. Results across all strains showed significant inhibitory effects that were dose dependent. The most sensitive strain was Staphylococcus aureus, which showed 44 mm inhibitory zone at a concentration of 81.38 mg/mL. The zones were somewhat smaller (16-28 mm) due to the complex cell wall structure of Gram-negative bacteria, however, the synthesized ZnO-NPs showed great effectiveness in combating these pathogens. The findings suggest that green-synthesized ZnO nanoparticles are a promising alternative for the treatment of nosocomial infections.