An Aptamer-Driven MWCNT-MoS2/Ag Nanohybrid Sensor for Sensitive Ciprofloxacin Sensing in Water
نویسندگان
1 Department of Organization, Management and Economics of Pharmacy and Clinical Pharmacy, Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan
2 Department of Infocommunication Engineering, Tashkent University of Information Technologies named after Muhammad Al-Khwarizmi, Tashkent, Uzbekistan
3 Technical Department, Urgench RANCH University of Technology, Urgench, Uzbekistan
4 Department of Prevention of Dental Diseases, Tashkent State Medical University, Tashkent, Uzbekistan
5 Department of Pediatrics No. 2, Bukhara State Medical Institute Named After Abu Ali Ibn Sino, Bukhara, Uzbekistan
6 Department of Neurology, Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
7 Department of Pediatrics, Faculty of Medicine, Samarkand Medical University, Samarkand, Uzbekistan
8 Department of Biology, Bukhara State University, Bukhara, Uzbekistan
9 Medical Chemistry Department, Andijan State Medical Institute, Andijan, Uzbekistan
10 Tashkent State Medical University, Tashkent, Uzbekistan
11 Department of Computer Engineering, Andijan State University, Andijan, Uzbekistan
12 Department of Mining work, Tashkent State Technical University named after Islam Karimov, Tashkent, Uzbekistan
13 Department of Obstetrics and Gynecology, Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
doi
10.22052/JNS.2026.03.046چکیده
The widespread presence of antibiotic residues in aquatic environments poses significant risks to ecosystems and public health, necessitating the development of sensitive and reliable detection methods. In this study, we report a novel electrochemical aptasensor for the selective and sensitive determination of ciprofloxacin in water samples, based on a multiwalled carbon nanotube-molybdenum disulfide/silver nanohybrid modified glassy carbon electrode. The MWCNT-MoS2/Ag nanocomposite was synthesized through a sequential approach involving carboxylation of MWCNTs, exfoliation and assembly of MoS2 nanosheets, and in situ reduction of silver nanoparticles. Comprehensive characterization using FESEM, FT-IR, and TGA confirmed the successful formation of the ternary nanohybrid with uniform distribution of components and enhanced thermal stability. A ciprofloxacin-specific aptamer was covalently immobilized onto the nanohybrid-modified electrode surface via amide bond formation, providing high specificity for target recognition. Under optimized conditions, the fabricated aptasensor exhibited a wide linear response ranging from 0.5 nM to 750 nM ciprofloxacin, with a low detection limit of 0.18 nM (S/N = 3). The sensor demonstrated excellent selectivity against potentially interfering substances, good reproducibility with relative standard deviation of 4.3%, and acceptable storage stability retaining 86.2% of initial response after four weeks. Furthermore, successful application to spiked environmental water samples with recoveries between 95.8% and 98.3% confirmed the practical utility of the proposed sensing platform for monitoring ciprofloxacin residues in real water matrices.
