فهرست نشریات علمی دسترسی آزاد ایران

Resistance to insecticides and inadequate compliance with the application protocols of topical pediculicides often lead to treatment failure. Essential oils or plant extracts function as effective and safe alternatives due to their low mammalian toxicity and great biodegradability. Aim of the study: This study aimed to determine the pediculocidal and larvicidal efficacy of synthetic silver nanoparticles (AgNPs) derived from the aqueous leaf extract of C. aurantium against the head louse Pediculus humanus capitis. Materials and methods: Samples of head lice were randomly collected from several locations throughout the limits of Tikrit Governorate. A study was conducted on the hair of 150 children, both male and female, aged 2 to 12 years, to detect head lice infestations and evaluate the prevalence of the parasite in the area. Subsequently, the detection of the COI gene via polymerase chain reaction. This study utilized silver nanoparticles synthesized from C.aurantium leaf extract via a green synthesis process, with characterization conducted using UV spectroscopy, Fourier Transform Infrared Spectroscopy Analysis (FTIR), scanning electron microscopy(SEM) and x-ray diffraction (XRD).  Result: The synthesis of AuNPs was confirmed via SPR as characterized by an absorption maximum at 435 nm. FTIR was used to estimate the biomolecular functional groups responsible for bioreduction of silver ions. XRD patterns of the AgNPs showed well-defined peaks at (211), (200), (220), (222) and (311) planes which provided an evidence to the face-centered cubic structure of silver crystallites. The particle sizes of the nanoparticles were in the range from from 23.06 to 38.48 nm and they have a nearly spherical shape, as indicated by SEM images. Nanoparticles derived from C.aurantium leaves had good outcomes at a concentration of 20 mg/L, resulting in greater fatality rates, followed by concentrations of 10 mg/L and subsequently 5 mg/L. Nanoparticles derived from C.aurantium leaves exhibited significant ovicidal action by postponing nymph emergence on the 6th and 14th days, ultimately inhibiting emerging entirely, akin to the standard. Conclusion: The green synthesis of nanoparticles considers an eco-environmental friend, safe, cheaper and easy to preparation. Synthesized AgNPs from C. aurantium extract have a high effect against adult insect and ovicidal activity by delaying the emergence of nymph.