Fe3O4 Nanoparticle-Enabled Zhimu-Huangbai Therapy for Type II Diabetes: A Self-Assembly Approach
نویسندگان
1 College of Health and Medical Technologies, National University of Science and Technology, Dhi Qar, Iraq
2 Al-Zahrawi University, Karbala, Iraq
3 INTI International University, 71800 Negeri Sembilan, Malaysia
4 Department of Ophthalmology, Samarkand State Medical University, Samarkand, Uzbekistan
5 Department of Pediatric Diseases, Termez Branch of Tashkent State Medical University, Termez, Uzbekistan
6 Department of Faculty Pediatrics, 2-camp, Tashkent State Medical University, Tashkent, Uzbekistan
7 Department of Dentistry and Otorhinolaryngology, Fergana Medical Institute of Public Health, Fergana, Uzbekistan
8 Faculty of Business and Communications, INTI International University, 71800 Negeri Sembilan, Malaysia.
9 Department of Pharmacy, Al-Turath University, Baghdad, Iraq
10 Department of Pharmacy, College of Pharmacy, Al-Nisour University, Baghdad, Iraq
11 Al-Hadi University College, Baghdad, Iraq
12 Department of Orthopedist Dentistry and Orthodontics, Bukhara State Medical Institute, Bukhara, Uzbekistan
13 Department of Orthopedist Dentistry and Orthodontics, Bukhara State Medical Institute, Bukhara, Uzbekistan
14 Department of Cardiologi, Andijan State Medical Institute, Andijan, Republic of Uzbekistan
doi
10.22052/JNS.2026.02.032چکیده
The integration of traditional herbal medicine with nanotechnology offers a promising strategy to overcome the limitations of conventional Type II Diabetes Mellitus (T2DM) therapies. This study developed a novel magnetite (Fe₃O₄) nanoparticle-enabled self-assembly platform for the co-delivery of the Zhimu-Huangbai (ZH) phytocomplex, a traditional herb pair with documented anti-diabetic properties. Highly crystalline, monodisperse Fe₃O₄ nanoparticles (~16.8 nm) were synthesized via a modified co-precipitation method. Subsequent self-assembly facilitated efficient loading of key bioactive compounds, berberine and mangiferin, with loading efficiencies of 78.4% and 65.1%, respectively. The resulting Fe₃O₄-ZH nanocomplex exhibited a distinct pH-responsive release, with significantly accelerated compound release (71–79% over 48 h) under simulated diabetic conditions (pH 5.5) compared to physiological pH (37–41%). In vitro evaluation using insulin-resistant HepG2 hepatocytes demonstrated that the nanocomplex significantly enhanced glucose uptake (142% of control) compared to the free extract (118%), at non-toxic concentrations. These findings indicate that the Fe₃O₄-based nanoplatform not only enhances the bioavailability of the ZH phytocomplex but also potentiates its therapeutic efficacy through improved cellular delivery and stimuli-responsive release. This work presents a rational, simplified approach to creating synergistic and targeted nanomedicines from multi-component botanical extracts for managing complex metabolic disorders.
