Eco-Friendly Synthesis and Characterization of Silver Nanoparticles Using Orange Peel Extract: Antibacterial Efficacy Against Clinical Isolates from Burn and Wound Infections
نویسندگان
1 Department of Medical Laboratory Techniques, College of Medical and Health Technologies, University of Bilad Al-Rafidain, Diyala, Iraq
2 Department of Medical Laboratory Techniques, College of Medical and Health Technologies, University of Bilad Al-Rafidain, Diyala, Iraq
doi
10.22052/JNS.2026.02.046چکیده
The increasing prevalence of antimicrobial resistance (AMR) in burn and wound infections necessitates alternative antimicrobial strategies. This study explores the green synthesis of silver nanoparticles (AgNPs) using orange peel extract as a sustainable alternative to combat pathogenic bacteria. AgNPs were synthesized using orange peel extract and characterized using UV-Vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR). Pathogenic bacteria were isolated from burn and wound samples, and the antimicrobial activity of the synthesized AgNPs was evaluated through inhibition zone measurements, and minimum inhibitory concentration (MIC). The synthesis was confirmed by a color change from orange to dark brown and further validated by UV-Vis spectroscopy, which showed a characteristic surface plasmon resonance peak at 418 nm. XRD analysis revealed that the AgNPs have a face-centered cubic structure, while TEM showed them to be polydisperse and spherical with an average size of 24.50 nm. FTIR identified various functional groups involved in the reduction and stabilization of AgNPs. The biosynthesized AgNPs exhibited significant antimicrobial activity against both Gram-positive and Gram-negative bacteria, including multidrug-resistant strains. The disk diffusion assay demonstrated clear zones of inhibition, with Staphylococcus aureus ATCC 33591 being the most sensitive strain. The minimum inhibitory concentration (MIC) for this strain was 25 µg/mL. The results highlight the potential of these green-synthesized AgNPs as effective antimicrobial agents against multidrug-resistant bacteria, offering a promising solution for addressing antimicrobial resistance challenges. This study contributes to the development of sustainable nanotechnology and provides insights into the applications of eco-friendly synthesized nanoparticles in healthcare.
