فهرست نشریات علمی دسترسی آزاد ایران

Porphyromonas gingivalis is a key pathogen in periodontitis that can form resistant biofilms, which complicate treatment. Conventional antimicrobial agents, such as chlorhexidine, are widely used but may be limited by microbial resistance and side effects. This study aimed to evaluate the antibiofilm efficacy of zinc nanoparticles (ZnNP) in combination with ultraviolet-visible (UV) photodynamic therapy and without UV, against biofilms formed by P. gingivalis. Clinical isolates of P. gingivalis were obtained from patients with periodontitis. Nanoparticles were synthesized using Pulsed Laser Ablation in Liquid (PLAL) and characterized by scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) analysis, UV-Vis spectroscopy, and Fourier-transform infrared (FTIR) spectroscopy. Antibiofilm activity was evaluated using microtiter plate biofilm assays before and after treatment. Statistical analysis was performed using the t-test, with significance set at (P < 0.05). The results showed that ZnNPs with (U.V) exhibited superior activity compared to ZnNPs alone, suggesting they are a promising alternative for periodontal treatments. ZnNPs with U.V demonstrated superior antibiofilm activity against P. gingivalis and presented a promising alternative to conventional antiseptics like chlorhexidine. These findings support further investigation of nanoparticle-based therapy as a promising approach for combating microbial resistance and biofilm-associated infections.