فهرست نشریات علمی دسترسی آزاد ایران

For thousands of years, people have utilized garlic (Allium sativum L) as a medicinal ingredient. The low solubility of garlic essential oil (GO) has restricted the usage of garlic despite its several biologically active components and its ability to function as a fungicide and antibacterial. Solid lipid nanoparticles (SLNs) are colloidal drug carriers that could be used to formulate GO in order to overcome its poor solubility and potentially be a good delivery system for GO.  This study was to determine the efficacy of GO-SLNs against three specific pathogens: Candida albicans, Escherichia coli, and Methicillin-Resistant Staphylococcus aureus (MRSA). High-shear homogenization and ultra-sonication methods were used to prepare GO-SLNs. The manufactured formulation’s encapsulation efficiency, loading capacity, and physicochemical characteristics were evaluated. Scanning electron microscopy (SEM) was used to analyze the morphology of GO-SLNs (SEM). Using GC/MS analysis, the chemical composition of the tested normal oil and GO-SLNs was assessed. In vitro studies were conducted to compare the antibacterial activity of GO-SLNs and regular GO. The particle size of GO-SLNs was 110.5±3 nm with PDI of 0.320±0.04 and zeta the potential was -36.6±0.5 mV. The majority of GO-SLNs had a less structured crystal structure, which contributed to improving the drug loading capacity. GO-SLNs had an encapsulation efficiency of 88±0.70% and a loading capacity of 0.56±0.0. According to the results, the GO-SLNs inhibited the growth of MRSA, E. coli, and Candida albicans by 74%, 54%, and 85%, respectively. In addition, GO-SLNs had greater antibacterial activity than regular GO. According to the study’s findings, changes need to be made to the loading capacity, encapsulation efficiency, and physicochemical characteristics. Our findings demonstrated that the SLNs were effective GO carriers for the control of bacterial and fungal diseases and that more research should be done on them.