Assessment of the Antibacterial and Antioxidant Efficacy of Nano-Chitosan Surface Derived from Fish Scale Waste

نویسندگان

1 Medical Devices Engineering Department, Al Rafidain University College, Baghdad, Iraq

2 Medical Laboratory Techniques Department, Al Rafidain University College, Baghdad, Iraq

3 Ministry of Higher Education and Science Research, Scientific Research Commission, Iraq

doi
10.22052/JNS.2026.01.039
چکیده

The biodegradability, biocompatibility, and low toxicity of chitosan make it an attractive polymer for use in drug delivery systems like nanoparticles. A Study Using XRD, FTIR, and SEM for Characterization. The observation of extremely wide peaks at 2θ= (10°, 20°, 26°) in X-ray diffraction studies of both pure and local extracted chitosan verifies the degree of crystallinity and structural conformance of the produced compound by means of the conformity of the angle values. The antimicrobial activity has been studied against different pathogenic bacterial isolates. By the well diffusion method, the highest inhibition zones were showed 30.5 mm, 26.2 mm, 23 mm against Klebsilla pneumoniae, Salmonella typhi and Staphylococcus aureus respectively, But, Candida albicans was appeared sensitivity with 17 mm at same concentration 30mg/mL of local extract chitosan compared with standard product. Antioxidant Activity have been studied free radical scavenging activities by ascorbic acid, Chitosan extract and stander chitosan, Chitosan extract showed radical scavenging (4 -16) % with Concentration (1- 2) mg/ml. Conclusion the antimicrobial activity against different microbial activity chitosan (well diffusion method), at (30 mg/mL) the bacteria appear different inhibition zone with high result Klebsiella Pneumoniae, and at (30 mg/mL) high Inhibition zone more than all antibiotic. The highest activity of local extracted chitosan against klebsiella pneumoniae. That indigenous extract may be utilized as an affordable antibacterial and antioxidant and can be made from fish and sold in an environmentally responsible manner.

کلیدواژه‌ها