Biosynthesis of Ag/ZnO Nanocomposite Using Ziziphus Jujuba Leaves Extract for Enhanced Synergistic Antibacterial Activity

نویسندگان

1 Ministry of Education, Baghdad, Iraq

2 Ministry of Education, Baghdad, Iraq

3 Department of Chemistry, College of Education for Pure Science- Ibn Alhaitham, University of Baghdad, Baghdad, Iraq

4 Department of Chemistry, College of Education for Pure Science- Ibn Alhaitham, University of Baghdad, Baghdad, Iraq

doi
10.22052/JNS.2026.01.046
چکیده

Over the past ten years, there has been a clear trend toward finding eco-friendly, long-lasting ways to make nanomaterials, especially composites with better properties. This study successfully synthesized silver/zinc oxide nanocomposite (Ag/ZnONC) utilizing an aqueous extract of Ziziphus jujuba leaves as a reducing agent and green filler, adhering to an eco-friendly approach. Spectroscopic and microscopic techniques were employed to characterize the biosynthesized nanocomposite. Fourier transform infrared spectroscopy (FTIR) elucidated the role of flavonoid compounds in Ziziphus jujuba extract during reduction and stabilization. Images from a field emission scanning electron microscope (FESEM) confirmed that mixing silver nanoparticles with zinc oxide nanoparticles made a hybrid and homogeneous structure. X-ray diffraction (XRD) spectroscopy also showed that the silver/zinc oxide nanocomposite was made correctly. This was shown by X-ray diffraction patterns that showed clear peaks for both materials. The wide diffraction peaks confirmed that the composite had a nanostructure. The Debye-Scherer equation says that the particle size of the composite component is about (45–55) nm. The synthesized nanocomposite consistently demonstrated significant efficacy against Enterococcus faecalis (Gram-positive) and Klebsiella pneumoniae (Gram-negative) bacteria, exhibiting a minimum inhibitory concentration (MIC) of 1.25% for both bacterial strains examined. The high effectiveness was due to a synergistic effect between the composite parts, which worked together in a number of complementary ways, such as producing reactive oxygen species (ROS), damaging the cell membrane in two ways, and interfering with cellular processes in the bacteria at the same time.The current study highlights the potential of Ziziphus jujuba leaves as an effective agent in the green synthesis of knowledgeable nanocomposites. Thus, offers the biosynthesized nanocomposite as a promising choice for biomedical applications throw the fight against multi-resistant microbes, supporting efforts to find green alternatives to traditional chemical methods.