فهرست نشریات علمی دسترسی آزاد ایران

The resistance to the most antibiotics increasing has led to an urgent need to alternative treatments development. Nanotechnology is a young scientific field offers promising solutions because of its potent impact of therapeutic as an alternative to most conventional therapies. In this study, copper nanoparticles (CuNPs) were synthesized via eco-friendly green synthesis of using Allium ampeloprasum extract as a natural reducing and stabilizing agent. The synthesized CuNPs were thoroughly characterized using multiple techniques including, Fourier Transform Infrared Spectroscopy (FTIR), Ultraviolet-Visible Spectroscopy (UV-Visible), Field Emission Scanning Electron Microscopy (FE-SEM), X-ray Diffraction (XRD), Zeta Potential Analysis, Dynamic Light Scattering (DLS) and cytotoxicity test. The characterization confirmed the formation of pure, stable nanoparticles with size22.5 nm and 21.1 nm to 44 nm in width, with others around and a zeta potential of -22.3 mV, indicating good colloidal stability. The antibacterial activity of CuNPs was evaluated against multidrug-resistant (MDR) bacterial strains Staphylococcus aureus. The nanoparticles exhibited maximum inhibition activity across a concentration range of 1.9–1000 µg/ml, with the maximum inhibition zone reaching to 27.4, 27 and 21mm for S. aureus and without cytotoxic effect on the normal human dermal fibroblasts neonatal. These findings highlight the promising antibacterial efficacy and biocompatibility of green-synthesized CuNPs, supporting their potential application in biomedical and antimicrobial therapies.