Application of Hydroxyapatite Nanoparticles Incorporated on AgFe2O4 Magnetic Nanoparticles in Dental Implant
نویسندگان
1 Department of Surgical Dentistry and Dental Implantology, Tashkent State Medical University, Tashkent, Republic of Uzbekistan
2 Department of Russian Language and Literature, Bukhara State Pedagogical Institute, Bukhara, Republic of Uzbekistan
3 Department of Agronomy, Navoi State Mining and Technological University, Navoi, Republic of Uzbekistan
4 Department of Propaedeutics of Internal Medicine, Bukhara State Medical Institute, Bukhara, Republic of Uzbekistan
5 Department of Medical Fundamental Sciences, Kimyo International University in Tashkent, Samarkand Branch, Samarkand, Uzbekistan
6 Urgench State University, Urgench, Uzbekistan
7 Department of Urology and Oncology, Fergana Medical Onstitute of Public Health, Fergana, Republic of Uzbekistan
8 Department of Obstetrics and Gynecology No.2, Samarkand State Medical University, Samarkand, Republic of Uzbekistan
9 Department of Pedagogy and Teaching languages, Urgut Branch of Samarkand State University named after Sharof Rashidov, Samarkand, Republic of Uzbekistan
10 Tashkent State Technical University, Tashkent, Republic of Uzbekistan
11 Department of Propaedeutics of Internal Diseases, Bukhara State Medical Institute, Bukhara, Republic of Uzbekistan
12 Department of Propaedeutics of Internal Medicine, Bukhara State Medical Institute, Bukhara, Republic of Uzbekistan
13 Department of Propaedeutics of Internal Medicine, Bukhara State Medical Institute, Bukhara, Republic of Uzbekistan
doi
10.22052/JNS.2026.01.081چکیده
This study presents the design and comprehensive evaluation of a novel “smart” nanocomposite coating. A core-shell architecture was engineered by integrating silver ferrite (AgFe₂O₄) magnetic nanoparticles with a bioactive hydroxyapatite (HAp) shell. The AgFe₂O₄ core provides both sustained antimicrobial silver ion release and magnetic responsiveness, while the HAp shell ensures osteoconductivity. A sequential solvothermal and wet-chemical precipitation synthesis yielded a well-defined HAp@AgFe₂O₄ nanocomposite, as confirmed by XRD, TEM, and FT-IR analysis. This material was processed into a uniform, adherent coating on Ti-6Al-4V substrates. In vitro studies demonstrated the coating›s high bioactivity, with a significant apatite-forming ability in simulated body fluid (mass gain of 1.52 mg/cm² after 21 days). The coating exhibited potent passive antibacterial efficacy (>88% reduction) against S. mutans and P. gingivalis. Crucially, exposure to an alternating magnetic field (350 kHz, 12 kA/m) triggered on-demand hyperthermia (ΔT = 12.5 °C), enhancing bacterial eradication to >97%. Furthermore, the coating supported human osteoblast (MG-63) proliferation and significantly increased alkaline phosphatase activity (1.6-fold vs. control), indicating enhanced osteogenic differentiation. The results confirm the successful creation of a dual-functional, magnetically responsive coating that synergistically promotes bone integration while offering a powerful, triggerable defense against infection, representing a significant advance towards intelligent dental implant surfaces.
