A Hybrid Platform Combining Nano Plasmonic U-turn Microfluidics and LIF Detection for High-Throughput Analysis of DNA Damage in Lymphocytes
نویسندگان
1 Institute of Laser for Postgraduate Studies, University of Baghdad, Iraq
2 Institute of Laser for Postgraduate Studies, University of Baghdad, Iraq
3 Biotechnology Research Center, University of Al-Nahrain, Baghdad, Iraq
doi
10.22052/JNS.2026.01.090چکیده
A microchip-based spiral system was fabricated with an integrated laser-induced fluorescence setup to study the size of genetic material preceding cancer onset. The chip was designed with three inlets: one for the isolated genetic material, the second for acridine orange dye, and the third for rhodamine dye at four different concentrations to determine the optimal energy transfer efficiency. In healthy cells, a single distinct emission peak appeared around 520 nm, while an additional peak extended toward 590 nm, alongside the green emission. The ratio of these peaks varied with the degree of structural degradation. When mixed with rhodamine, the 1:1.5 ratio produced the best energy transfer before photobleaching occurred. The gold coating, leveraging a plasmonic effect, and a smooth laminar flow significantly boost the signal clarity and minimized the need for reagents. This work represents a parallel step toward early cancer detection when conventional methods fail, reducing both the physical and psychological burden experienced by patients during routine examinations.
