فهرست نشریات علمی دسترسی آزاد ایران

The purpose of this study was to look at the direct and indirect cytotoxic effects of two universal dental adhesive agents combined with silver colloidal dispersion on a human gingival fibroblast cell. Peak universal (Ultradent, USA) and Optibond Universal (Kerr, America) commercial dental adhesive agent systems were infused with 8% by mass of colloidal dispersion containing silver nanoparticles (AgNps). Adult rabbits were used to create a cell line of human gingival fibroblast cells. The cytotoxic activity of four adhesive agent groups on fibroblast-like cells was investigated using two cytotoxic assays: GA: Peak Universal (control), GB: Peak Universal (8% AgNps incorporated), GC: Optibond universal (control), and GD: Optibond (8% AgNps incorporated). From the adhesive agent groups, forty adhesive agent samples (5 x 1 mm discs) were manufactured and utilized for the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test, and 32 discs were used for the High-content screening (HCS) assay. The cytotoxic assay results revealed that all adhesive agents examined had a high degree of cytocompatibility. However, the 8% incorporated adhesive agent Groups (GB and GD) had much lower cytotoxicity than their controls. Furthermore, groups GC and GD had significantly better cytocompatibility than groups GA and GB. In comparison to their control groups, the incorporation of 8% by mass colloidal dispersion of silver nanoparticles greatly improved the biocompatibility of the evaluated universal adhesive agents.