Study the Effect of CuO Nanoparticle on the Immunohisto-Chemical Expression of Cadherin in the Renal Parenchyma of Male Mice
نویسندگان
1 Department of Physiology and Medical Physics, College of Medicine, Al Nahrain University, Baghdad, Iraq
2 Department of Physiology and Medical Physics, College of Medicine, Al Nahrain University, Baghdad, Iraq
3 Department of Anatomy, College of Medicine, Al Nahrain university, Baghdad, Iraq
doi
10.22052/JNS.2025.04.025چکیده
The kidney cortex, containing renal corpuscles and convoluted tubules, is particularly vulnerable. CuO NPs are widely used in industrial and consumer products, raising concerns about their impact on vital organs. Cadherins, transmembrane proteins essential for cell adhesion and tissue integrity, can reflect cellular damage when their expression changes. In this study, sixty Swiss rats were divided into four groups. Group D served as control, while Groups A, B, and C received intraperitoneal CuO NP doses (0.6, 0.45, and 0.3 mg/kg/day) for 45 days. Histopathological and immunohistochemical analyses revealed immune cell infiltration and congested blood vessels in treated groups, especially at higher doses. E-cadherin expression increased with CuO NP dosage, visible as dark brown staining in renal tissue using anti-cadherin IHC and image analysis software. This suggests CuO NPs have an affinity for E-cadherin in nephron epithelium. Their 45–50 nm size may disrupt the renal filtration barrier, typically 30–40 nm wide, impairing glomerular function and inducing apoptosis.
