Use of PCR Technology in Diagnosis of Pseudomonas Aeruginosa Isolated from Filters of Factory Health Water Production and Analysis of Antibacterial Activity of Iron Oxide Nanoparticles against Resistance Bacteria
نویسندگان
1 Commodity Evaluation and Service Performance Department, Market Research and Consumer Protection Center, University of Baghdad , Baghdad, Iraq
2 Commodity Evaluation and Service Performance Department, Market Research and Consumer Protection Center, University of Baghdad , Baghdad, Iraq
3 Commodity Evaluation and Service Performance Department, Market Research and Consumer Protection Center, University of Baghdad , Baghdad, Iraq
doi
10.22052/JNS.2025.04.040چکیده
Most species of Pseudomonas aeruginosa can be detected in water treatment systems, hospital environments. The result of court study found four isolates of P. aeruginosa from 30 sample of health waterin Baghdad City. PCR result show four isolates were producing gyr B gene with amplified size 367 bp. Results of the FTIR analysis showed that Iron Oxide Nanoparticles had six bands at (3253, 2941, 1477, 1001, 685 and 587) cm-1. AFM analysis shows a three-dimensional and two-dimensional image for Fe3O4 NPs with molecular clusters was up 15.60 nm and peaks 39. Results of SEM and TEM shows inclusion of numerous nanoparticles with spherical Fe3O4 nanostructures and size of particles 30 nm. Fe3O4 NPs resulted in 9 mm inhibition at a concentration of 10 mg/ml, which increased to 16 mm with a concentration of 15 mg/ml, respectively. The study aimed to detection of Pseudomonas aeruginosa from Filters of Health Water then used iron oxide nanoparticles against Pseudomonas aeruginosa.
