Fe3O4-Polyvinylpyrrolidone-Decorated on Graphene Oxide Nanosheets for Fast Detection of Trace Protein
نویسندگان
1 Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
2 Alfraganus University, Tashkent, Uzbekistan
3 Chirchik State Pedagogical University, Chirchik , Uzbekistan
4 Bukhara State Medical Institute, Bukhara, Uzbekistan
5 Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
6 Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
7 Urgench State University named after Abu Rayhan Beruni, Urgench, Uzbekistan
8 Tashkent State University of Economics, Tashkent, Uzbekistan
9 Tashkent State Medical University, Tashkent, Uzbekistan
10 Tashkent Institute of Irrigation and Agricultural Mechanization Engineers National Research University, Tashkent, Uzbekistan
11 Bukhara State Pedagogical Institute, Bukhara, Uzbekistan
12 Samarkand Regional Center for Pedagogical Skills, Samarkand, Uzbekistan
13 Bukhara State University, Bukhara, Uzbekistan
doi
10.22052/JNS.2025.04.047چکیده
We reported a magnetically recoverable Fe3O4–PVP–GO nanocomposite designed for rapid, selective detection of trace proteins in complex matrices, addressing the persistent challenges of capture specificity, enrichment without dilution, and low-cost readout in point-of-care settings. The synthesis integrates three components: Fe3O4 magnetic cores for fast, quantitative magnetic separation; polyvinylpyrrolidone (PVP) to stabilize and present a hydrophobic/hydrogen-bonding interface; and graphene oxide (GO) to provide a high-surface-area scaffold with abundant π–π and electrostatic interaction sites. The resulting Fe3O4–PVP–GO nanocomposite achieves near-quantitative recovery (>99.5%) within 30 seconds under a modest magnetic field (1.2 T), enabling rapid preconcentration from 10 mL samples and minimizing matrix carryover. Characterization by FE-SEM, FT-IR, XRD, VSM, and TGA confirms preserved magnetite crystallinity, substantial surface area, and robust integration of components without covalent grafting, indicating a predominantly supramolecular assembly driven by π–π interactions and hydrogen bonding. Binding to model trace proteins (BSA) demonstrated sub-attomolar detection limits (LOD ≈ 0.45 fM) with a three-log dynamic range (0.1–100 ng mL−1) and inter-day RSD ≤ 3.9%. Real-sample analyses in human serum, skimmed milk, and river water yielded recoveries of 93–104% with minimal matrix effects. Reusability over fifteen cycles showed <4% loss in binding capacity. This work establishes a robust, low-cost platform for rapid on-site protein quantification with high fidelity and scalability.
