Green Synthesis of Cobalt Oxide Nanoparticles Using Rosmarinus Officinalis L. Leaf Extract: Characterization and Evaluation of Enzyme Inhibition and Antimalarial Potential
نویسندگان
1 Department of Chemistry, College of Science, University of Babylon, Iraq
2 Research and Development Department, Hilla Textile Factory, The Company for Textile Industries, Iraq
3 Al-Mussaib Technical College, Al-Furat Al-Awsat Technical University, Iraq
4 Department of Chemistry, College of Science, University of Basrah, Iraq
doi
10.22052/JNS.2025.04.050چکیده
The green synthesis of cobalt oxide nanoparticles (Co₃O₄-NPs) using Rosmarinus officinalis L. leaf extract offers a sustainable and biocompatible route for nanomaterial production. GC-MS analysis of the aqueous extract identified 24 phytochemicals, including reducing agents (e.g., octadecanal, bicyclo[3.1.1]heptane-3-one) and stabilizers (e.g., cis-vaccenic acid, α-pinene), which facilitated Co²⁺ reduction and nanoparticle capping via electron donation and steric hindrance. The synthesized Co₃O₄-NPs were characterized by UV-Vis, FT-IR, SEM, EDX, and XRD, revealing a crystalline monoclinic structure (30–54 nm) with high purity. The nanoparticles exhibited dose-dependent acetylcholinesterase (AChE) inhibition (70% at 1000 μg/mL), attributed to their interaction with the enzyme’s active site. Notably, Co₃O₄-NPs suppressed beta-hematin formation by 80% at 50 μg/mL, outperforming chloroquine, likely due to heme-binding surface properties. This dual functionality—enzyme inhibition and antimalarial activity—positions R. officinalis-derived Co₃O₄-NPs as promising candidates for neurodegenerative and antiparasitic therapies.
