Determination of Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of Zinc Oxide Nanoparticles from Internal Cavity of Dental Implant and Natural Teeth (in vitro study)
نویسندگان
1 College of Dentistry, The Islamic University, Najaf, Iraq
2 Department of Oral and Maxillofacial Surgery, College of Dentistry, University of Kufa, Najaf, Iraq
3 Department of Pediatric, Orthodontics & Preventive Dentistry, College of Dentistry, University of Kufa, Najaf, Iraq.
doi
10.22052/JNS.2025.04.055چکیده
Nanotechnology characterizes a new knowledge that potentials to deliver a wide range of uses and improved technologies for biological and biomedical applications. Nanotechnology permits synthesis of materials that have structure is less than 100 nanometers. The present work revealed the determination minimum inhibitory concentration and maximum bactericidal concentration of zinc oxide nanoparticles for aerobic and anaerobic bacteria isolated from internal cavity of the implant and sulcus of the natural teeth. Bacteria were isolated from internal hole of the implant after 90 days from surgical placement for 16 dental implant and sulcus of natural teeth for 6 female age range 30-44years. Different concentrations of ZnO NPs were prepared in solvent (water 3:1 ethanol) and mixed with brain heart infusion agar all the experiments were conducted in vitro. Agar dilution method was used to study the minimum inhibitory concentration and minimum bactericidal concentration and MBC for tested bacteria. The MIC for aerobic and anaerobic bacteria isolated from internal cavity of the implant and the sulcus of the natural teeth maximum plate was (0.05mg/ml) and (0.08mg/ml) respectively while the MBC for both bacterial groups were (0.08mg/ml) and (0.1mg/ml) respectively. This study exposed that zinc oxide nanoparticles were able to inhibit and kill the aerobic and anaerobic bacteria.
