Synthesis and Characterization of Novel Biocompatible MgO-CaO Polycaprolactone-Carbon Nanotubes (MgO–CaO–PCL–CNT) Nanocomposites for Enhancement of Efficiency Regeneration for Bone Tissue Engineering
نویسندگان
1 Samarkand State University named after Sharof Rashidov, Uzbekistan
2 Navoi State Mining and Technological University, Navoi, Uzbekistan
3 Urgench State University, Uzbekistan
4 Samarkand State University of Veterinary Medicine, Livestock and Biotechnologies, Samarkand, Uzbekistan
5 Samarkand State Medical University, Samarkand, Uzbekistan
6 Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
7 Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
8 Tashkent State Medical University, Tashkent, Uzbekistan
9 Tashkent Institute of Irrigation and Agricultural Mechanization Engineers National Research University, Tashkent, Uzbekistan
10 Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
11 Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
12 Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
13 Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
doi
10.22052/JNS.2025.04.059چکیده
In this research, we reported the design, synthesis, and comprehensive characterization of biocompatible MgO–CaO–PCL–CNT nanocomposites for bone tissue engineering. A four-step sequential in-situ strategy enabled homogeneous dispersion of nanoscale MgO (20–40 nm) and CaO (30–50 nm) within a crosslinked poly(ε-caprolactone) (PCL) matrix while integrating carboxylated CNTs (CNT–COOH) via APTES-mediated silanization and EDC/NHS coupling to promote covalent interfacial bonding. Composite fabrication combined melt processing (60–80 °C above PCL melting) and solvent casting, producing dense films and porous scaffolds with interconnected porosity achieved through salt leaching (porosity 40–60%; pore sizes 200–600 μm). Three representative compositions (25/25/40/10, 40/40/15/5, 60/60/15/5 by wt%) maintained polymer integrity, with FE-SEM confirming uniform ceramic dispersion and an integrated CNT network. TGA indicated filler loadings of 28–33 wt% with residual inorganic/CNT content stable to 800 °C in air, while DTA showed subtle CNT-related exotherms near 320–360 °C. XRD preserved MgO/CaO crystallinity and PCL identity throughout processing, and FTIR corroborated interfacial coupling without new phase formation. In vitro, composites exhibited high osteoblast viability (>92%), low cytotoxicity, and upregulation of osteogenic markers (ALP, RUNX2) within 7–14 days, alongside controlled protein adsorption (0.42–0.66 μg cm−2 at 1 h; 0.58–0.82 μg cm−2 at 4 h). Collectively, MgO–CaO–PCL–CNT nanocomposites provide mechanical robustness, tailored bioactivity, and architectures conducive to bone regeneration, warranting further in vivo evaluation toward clinical translation.
