Application of CuFe2O4 Nanoparticles as an Effective Nanocarrier in Antibacterial Efficacy of Smart Drug Delivery System for Encapsulation of Gentamycin and Chloramphenicol
نویسندگان
1 Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
2 Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
3 Samarkand Campus, University of Economics and Pedagogy, Uzbekistan
4 Jizzakh Polytechnic Institute, Jizzakh, Uzbekistan
5 Jizzakh Branch of the National University of Uzbekistan, Jizzakh, Uzbekistan
6 Samarkand State Medical University, Samarkand, Uzbekistan
7 Urgench State University, Khorezm, Uzbekistan
8 Tashkent State Medical University, Tashkent, Uzbekistan
9 Bukhara State University, Bukhara, Uzbekistan
10 Bukhara State Pedagogical Institute, Bukhara, Uzbekistan
11 Mamun University, Khorezm, Uzbekistan
12 Urganch Innovation University, Urgench, Uzbekistan
13 Tashkent State University of Economics, Tashkent, Uzbekistan
doi
10.22052/JNS.2025.04.066چکیده
Super-paramagnetic CuFe₂O₄ (18 ± 3 nm, 39 emu g⁻¹) was synthesised via CTAB-directed co-precipitation; antibiotics were surface-loaded under mild aqueous conditions to yield GT@CuFe₂O₄ (17.3 wt % gentamycin) and CM@CuFe₂O₄ (9.1 wt % chloramphenicol). Bactericidal activity was quantified by broth micro-dilution and drop-plate enumeration against ATCC 25922 and 29213 strains; magnetic guidance (1.3 T) and release kinetics (pH 5.5/7.4) were monitored by ICP-OES and HPLC-UV. GT@CuFe₂O₄ eradicated planktonic E. coli at 15.6 µg mL⁻¹ (0.28 µg mL⁻¹ released drug), whereas CM@CuFe₂O₄ achieved 31.3 µg mL⁻¹ against S. aureus (2.8 µg mL⁻¹ released drug); both values matched free-antibiotic MICs yet required 4- to 5-fold lower antibiotic doses. A 30-min magnetic exposure halved the effective MIC for E. coli and enabled > 95 % particle recovery within 60 s. Zero-order release (0.12 µg mL⁻¹ h⁻¹) persisted for 24 h at pH 5.5, mirroring biofilm acidification kinetics. CuFe₂O₄ nanocarriers act as redox-silent, magnetically addressable depots that amplify aminoglycoside/amphenicol potency while reducing systemic load, offering a clinically translatable strategy for precision antibacterial therapy.
