فهرست نشریات علمی دسترسی آزاد ایران

This research examined the identification of biogenic selenium nanoparticle (SeNPs) synthesized with lemon peel extract, the antimicrobial and antimicrobial biofilm inhibition test conducted on Salmonella enterica isolates, the prepared surface confirmed by XRD, EDX and FESEM techniques. The selective media committed in identification of the Salmonella isolates were SS and XLD whereby they exemplified normal colony morphologies characteristics of living organisms producing sulfur (hydrogen sulfide is produced by Salmonella species). Biochemical identification was also authenticated using Vitek2 Automated analysis and the probabilities given are similar to the Salmonella species identification that is 94-99% and were confirmed using molecular tools that is gel electrophoresis and NCBI BLAST analysis where high percentages of identity were shown 93.41% to 99.75% to Salmonella enterica subsp. enterica. The results of antibiotic susceptibility testing revealed high uncertainty in resistance pattern as far as some of the isolates were multiple-drug resistant (MDR), which indicates the increasing problem of antibiotic resistance related to Salmonella. Antimicrobial properties of biogenic SeNPs were also determined and it was identified that antimicrobial action of the nanomaterials was dose-dependent with minimum inhibitory concentration (MIC) of 32 µg/mL. Furthermore, the SeNPs had a high inhibitory effect on the biofilm formation that was also synergistic once combined with tetracycline. These data indicate that biogenic SeNPs are promising antimicrobial agents and add-ons to conventional antibiotics against Salmonella infection and biofilm-related resistance.