Application of Fe3O4-Celloluse Acetate Supported on Hydroxyapatite Multi-Walled Carbon Nanotubes (Fe3O4-CA-HA-MWCNTs) in Bone Tissue Engineering
نویسندگان
1 Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Bukhara, Uzbekistan
2 Jizzakh branch of the National University of Uzbekistan, Jizzakh, Uzbekistan
3 Jizzakh branch of the National University of Uzbekistan, Jizzakh, Uzbekistan
4 Tashkent State Medical University, Tashkent, Uzbekistan
5 Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Bukhara, Uzbekistan
6 Mamun university, Urgench city, Uzbekistan
7 Nukus State Pedagogical Institute named after Ajiniyaz, Nukus, Uzbekistan
8 Tashkent State Technical University, Tashkent, Uzbekistan
9 Kimyo International University in Tashkent, Samarkand 140 100, Uzbekistan
10 Chirchik State Pedagogical University, Chirchik, Uzbekistan
11 Tashkent State Medical University, Tashkent, Uzbekistan
12 Kokand State University, Fergana, Uzbekistan
13 Urgench State University, Urgench, Uzbekistan
doi
10.22052/JNS.2025.04.086چکیده
Critical-sized long-bone defects still resist endogenous healing because contemporary grafts fail to couple mechanical integrity with spatiotemporally resolved bio-electrical cues. We report a quaternary construct, Fe₃O₄-cellulose-acetate-hydroxyapatite-multi-walled carbon nanotubes (Fe₃O₄-CA-HA-MWCNTs), that unites load-bearing modulus, magnetic actuation, osteoinduction and imaging visibility in a single injectable bead. Hybrid microfibres (187 ± 23 nm) were first generated by co-electrospinning cellulose-acetate doped with 24 wt % super-paramagnetic Fe₃O₄ (8–10 nm) and 28 wt % HA-decorated MWCNTs; alkaline regeneration exposed cellulosic –OH, yielding 78 % open porosity and 185 kPa compressive strength. Remote magnetic stimulation (0.15 T, 50 Hz, 30 min day-1) elevated ALP 1.8-fold and mineral deposition 2.1-fold versus static controls without compromising viability (> 90 %). Antibacterial assays showed 2.9 mm inhibition zones against E. coli at 5 wt % Ag with no cytotoxicity. Enzymatic degradation released 24 % mass within 10 days while the HA–MWCNT core remained intact (Raman D/G 0.84). In a 5 mm rat femoral defect, 76 ± 4 % radiographic bridging was achieved at 8 weeks versus 18 ± 3 % for empty defects (p < 0.001) with a histological score of 1.1 ± 0.2, confirming low inflammation and mature trabeculae. The study delivers an instructive, magnetically responsive scaffold that begins as an agricultural side-stream and finishes as cortical bone, offering a clinically translatable route for load-bearing segmental repair.
