Fe3O4-ZnO Incorporated in Chitosan-Carbon Nanotubes (Fe3O4-ZnO-CHIT-CNT) as a Hybrid Organic Inorganic Biodegradable Nano-composites for Application in Bone Tissue Engineering
نویسندگان
1 Termez State University of Engineering and Agrotechnology, Termez, Uzbekistan
2 Tashkent State Technical University named after Islam Karimov, Tashkent, Uzbekistan
3 Samarkand State University named after Sharof Rashidov, Samarkand, Uzbekistan
4 Bukhara State Medical Institute, 23, Gijduvanskaya str, Bukhara city, 200118, Uzbekistan
5 Urgench State University, Uzbekistan
6 Samarkand State Medical University, Samarkand, Uzbekistan
7 Tashkent State Technical University named after Islam Karimov, Tashkent, Uzbekistan
8 Almalyk branch of Tashkent State Technical University, Uzbekistan
9 Professional Development Center for Medical Workers, Tashkent, Uzbekistan
10 Jizzakh State Pedagogical university, Uzbekistan
11 Bukhara State Medical Institute, 23, Gijduvanskaya str, Bukhara city, 200118, Uzbekistan
12 Tashkent Institute of Irrigation and Agricultural Mechanization Engineers National Research University, Tashkent, Uzbekistan
13 Termez State Pedagogical Institute, Termez, Uzbekistan
doi
10.22052/JNS.2025.03.053چکیده
Bone tissue engineering seeks innovative solutions to address critical-sized bone defects that surpass the body’s natural regenerative capacity. In this study, we developed a novel hybrid nanocomposite integrating Fe₃O₄ and ZnO nanoparticles within a chitosan-carbon nanotube (CNT) matrix to serve as an effective scaffold for bone regeneration. The synthesis involved functionalization of CNTs, formation of inorganic nanostructures, and their precise incorporation into a biodegradable chitosan matrix, resulting in a hierarchically structured, biocompatible material. Extensive characterization confirmed the successful embedding of inorganic phases and the preservation of crystalline integrity, with FE-SEM revealing uniform nanostructure distribution, FT-IR identifying chemical interactions, and XRD confirming phase purity. Biological assessments demonstrated high cytocompatibility and enhanced osteogenic activity, supported by cell viability, proliferation, ALP activity, and mineralization results. Mechanical testing indicated excellent load-bearing properties, while in vitro bioactivity assays showed significant apatite formation. The scaffold exhibited sustained biodegradability and maintained a physiological pH during degradation. These findings suggest that Fe₃O₄-ZnO-CHIT-CNT nanocomposites possess promising potential as multifunctional, biodegradable scaffolds for effective bone tissue regeneration, paving the way for future clinical applications in regenerative medicine.
