Design and Application of Responsive and Smart Gold Nanoparticles Distributed on L-histidine Supported on Fe3O4 (Au-LH-Fe3O4) for Advanced Biomedical Diagnostics of Breast Cancer Cells
نویسندگان
1 Department of Anatomy and Clinical Anatomy (OSTA), Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Republic of Uzbekistan
2 Department of Family Medicine № 2, Clinical Pharmacology, Tashkent State Medical University, Republic of Uzbekistan
3 Department of Family Medicine № 2, Clinical Pharmacology, Tashkent State Medical University, Republic of Uzbekistan
4 Department of Therapeutic stomatology, Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Republic of Uzbekistan
5 Department of Hospital Therapy (Laboratory), Fergana Medical Institute of Public Health, Fergana, Republic of Uzbekistan
6 Social and Humanities Departments, Urganch Innovation University, Urgench, Republic of Uzbekistan
7 Department of Internal Diseases No. 1 named after N.A. Abdullaev, PhD in Medical Sciences, Samarkand state medical University, Samarkand, Uzbekistan
8 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Andijan State Medical Institute, Republic of Uzbekistan
9 Department of Internal diseases, Bukhara State Medical Institute named after Abu Ali ibn Sino. Bukhara, Uzbekistan
10 Department of Traditional Medicine, Occupational Diseases and Allergology, Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan
11 Department of Chemistry, Jizzakh State Pedagogical University, Jizzakh, Republic of Uzbekistan
12 Tashkent State Technical University, Uzbekistan
13 Department of Biology, Urgench State University, Uzbekistan
doi
10.22052/JNS.2025.03.057چکیده
This work presents the rational design, synthesis, and application of a multifunctional nanoplatform, designated Au-LH-Fe₃O₄, for enhanced biomedical diagnostics of breast cancer cells. The nanocomposite combines superparamagnetic Fe₃O₄ nanoparticles, functionalized with L-histidine for improved biocompatibility and surface chemistry, with the in-situ growth of gold nanoparticles, which impart excellent optical properties and facilitate molecular recognition. The synthesis involved controlled co-precipitation of Fe₃O₄, covalent attachment of L-histidine through chelation interactions, followed by the reduction of gold ions on the modified surface, producing uniformly distributed gold nanoparticles confirmed via advanced microscopy and spectroscopic techniques. Magnetic measurements demonstrated superparamagnetic behavior with a high saturation magnetization (~45 emu/g), essential for rapid magnetic separation and targeted delivery. Spectroscopic analyses evidenced the preservation of functional groups and successful nanoparticle deposition. The nanocomposite exhibited remarkable stability, dispersibility, and biocompatibility. Importantly, the Au-LH-Fe₃O₄ platform demonstrated high specificity and sensitivity in detecting breast cancer cell lines (MCF-7 and SK-BR-3), achieving detection limits in the nanogram range. The facile synthesis, combined with its multi-functionality, advocates this nanoplatform as a promising candidate for early, label-free cancer diagnostics and potential theranostics, supporting the advancement toward personalized medicine.
