فهرست نشریات علمی دسترسی آزاد ایران

The process of producing zinc oxide nanoparticles using Lactobacillus casei and their subsequent analysis of properties. Structure and morphological measurements include research that includes measurements with X-ray diffraction, as well as the use of electron microscopy (SEM) and optical measurements. Optics measurements include UV-Vis diffusion reflection measurements and Fourier transformation infrared measurements (FTIRs). In this study, PHA was extracted from L. casei, and its characteristics were determined using UV spectrophotometers. The techniques used in this study are FTIR, GC-MS, DSC, and TGA. The blue Nile was used to detect PHA. In addition, the study examined the cytotoxicity of zinc oxide nanoparticles (ZnONPs) and nanobiocomposites ZnO/PHA in normal cell lines. The study found that WRL cells were exposed to ZnO extract and control substances for 24 hours at a dose of 50 to 400 ml. The biosynthesis of zinc oxide yields the lowest cell performance (72.84%), with a concentration of 400g/ml. In contrast, the Ambiguous’s control group had a cell viability of 86.17 percent at the same concentration. However, the results show that WRL cells are used in the same amount of ZnO / PHA nano biocomposite extract (Z2) and ambiguous substances used as control when incubated. The ZnO/PHA nanobiocomposite extracts have the lowest cell survival rates at 400 g/ml and have a recorded 76.20% percentage. Compared to the Ambiguous, the cell survival rate was 91.86 percent. At the same concentration, ZnO and ZnO/PHA show mild cell toxic effects in normal cells. The study studied the cytotoxic effect of ZnONPs) and nanobiocomposites (ZnO/PHA) in cancer cell lines. The results showed that MCF-7 cells were exposed to ZnO extracts at a dose of 50 to 400 mg/ml for 24 hours, with an uncertain control. Zinc oxide extraction showed the lowest MCF-7 cell viability at 400 g/ml with a percentage of 43.56 percent. In comparison, the cell viability of water-represented control groups was 79.89% at the same concentration. However, this showed that MCF-7 cells incubated at the same dose with ZnO/PHA nanocomposite extract (Z2) did not have conclusive results compared to the control. ZnO/PHA extracts have the lowest MCF-7 cell survival rate with a concentration of 400 g/ml and a percentage of 43.90%. In contrast, the same concentration of control groups treated with water had a cell survival rate of 85.24 percent. The study showed significant cytotoxicity of ZnO and ZnO/PHA on cancer cells, as opposed to normal cells.