Enhanced Wound Repair in Rats via a Chitosan-Based Transdermal Patch with Selenium Nanoparticles and Platelet-Rich Plasma
نویسندگان
1 Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Department of Pharmaceutics, College of Pharmacy, Ahl AL-Bayt University, Karbala, Iraq
3 Department of Anesthesia Techniques and Intensive Care, Al-Taff University College, Karbala, Iraq
4 Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran
5 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
6 Department of Pharmaceutics, College of Pharmacy, Al-Zahraa University for Women, Karbala, Iraq
7 Department of Pathobiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
8 Department of Veterinary Surgery and Radiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
9 Department of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
doi
10.22052/JNS.2025.02.035چکیده
The process of wound healing is complex and can be impaired by infection and inflammation. Chitosan (CS), selenium nanoparticles (SeNPs), and platelet-rich plasma (PRP) have individually shown promise in promoting wound healing due to their regenerative and antioxidant properties. However, their combined use in a transdermal patch for synergistic effects remains underexplored. This study aimed to develop and evaluate a novel chitosan-based transdermal patch incorporating SeNPs and PRP to explore their collective effectiveness in facilitating wound healing in a rat model. Seventy-five male Wistar rats with 1.5 × 1.5 cm² wounds were divided into five groups: control, free patch, CS/SeNPs, CS/PRP, and CS/SeNPs/PRP. Patches were prepared by dissolving chitosan in acetic acid, adding propylene glycol, SeNPs, and PRP, and then freeze-drying. Wound healing was assessed on days 3, 7, and 21 using histopathology, cytokine expression, and fluorescent immunohistochemistry for vascular growth factors. The CS/SeNPs/PRP patch significantly outperformed other treatment groups in terms of wound closure rate (p < 0.05). Histological analysis revealed enhanced re-epithelialization, collagen deposition, and angiogenesis. The observed reduction in inflammatory cytokine levels, specifically TNF-α and IL-6, suggests an anti-inflammatory effect contributing to the overall improvement in wound healing outcomes. This novel transdermal patch combining the beneficial properties of CS, SeNPs, and PRP offers a promising therapeutic approach for promoting wound healing through accelerated tissue regeneration and modulation of inflammation.
