Multi-Amine Decorated with Gold Nanoparticles Supported on Multi-Walled Carbon Nanotubes (CNT-CPTMS-PEHA-Au) as Novel Nanocomposites for Investigation of Photothermal Chemotherapy
نویسندگان
1 Warka University College, Iraq
2 College of Pharmacy, Ahl Al Bayt University, Kerbala, Iraq
3 Department of Dentistry, Al-Manara College For Medical Sciences, Maysan, Iraq
4 Department of Nursing, Al-Zahrawi University College, Karbala, Iraq
5 Ferghana Medical Institute of Public Health, Republic of Uzbekistan
6 Department of Obstetrics-Gynecology №2, Samarkand State Medical University, Uzbekistan
7 Department of Fruits and Vegetables At the Urganch State University, Uzbekistan
8 Department of Biochemistry, NCJSC Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan
9 Department of Clinical Pharmacy, College of Pharmacy, University of Al-Ameed, Karbala, Iraq
10 College of Health and Medical Technologies, National University of Science and Technology, Dhi Qar, Iraq
11 Mazaya University College, Iraq
12 Department of “Nuclear Medicine and Medical Radiology”, Bukhara State Medical Institute, Bukhara, Uzbekistan
13 Tashkent State Technical University named after Islam Karimov, Uzbekistan
doi
10.22052/JNS.2025.02.036چکیده
In this study, a novel nanocomposite, CNT-CPTMS-PEHA-Au, was synthesized through a multi-step surface functionalization strategy aimed at enhancing photothermal chemotherapy efficacy. Multi-walled carbon nanotubes (MWCNTs) were first oxidized to introduce functional groups, followed by silanization with 3-chloropropyltrimethoxysilane (3-CPTMS) and subsequent attachment of pentaethylenehexamine (PEHA) to graft amino functionalities. Gold nanoparticles (AuNPs) were then uniformly deposited onto the functionalized CNT surface via in situ reduction of HAuCl₄ with sodium borohydride, resulting in a stable nanocomposite exhibiting well-dispersed AuNPs approximately 40–50 nm in size. Characterization through FE-SEM and FT-IR confirmed the morphological integrity, successful surface modifications, and gold loading. The nanocomposite demonstrated rapid and stable in vitro photothermal conversion, reaching a maximum temperature of 47.8 °C within 10 minutes under 808 nm laser irradiation at 1.5 W/cm², with excellent thermal stability over consecutive cycles. Biological evaluation using MCF-7 cells revealed significant temperature-dependent cytotoxicity, with up to 67% cell death at 50 μg/mL post-irradiation. These results underscore the potential of CNT-CPTMS-PEHA-Au as an efficient, stable, and targeted platform for minimally invasive photothermal cancer therapy, while highlighting challenges related to in vivo translation and scalable synthesis. Future studies should focus on targeting specificity and biocompatibility enhancements for clinical viability.
