Converting the Hematotoxicity of Doxorubicin to Antioxidant on Blood by Coating with PEGylated Chitosan Nanoparticles
نویسندگان
1 Department of Pathological Analysis Techniques, College of Health and Medical Techniques, Middle Technical University, Baghdad, Iraq
2 Department of Pathological Analysis Techniques, College of Health and Medical Techniques, Middle Technical University, Baghdad, Iraq
3 Department of Pathological Analysis Techniques, College of Health and Medical Techniques, Middle Technical University, Baghdad, Iraq
doi
10.22052/JNS.2025.01.008چکیده
Doxorubicin (DOXO) is a potential antineoplastic agent used for treating several tumors. It has severe side effects on the hematological profile. Chitosan is an effective nano-carrier for delivering therapies. This study aims to convert the oxidative effect of DOXO to an antioxidant effect by coating it with chitosan nanoparticles (CsN). Three steps prepared this; (1) the CsN preparation, (2) the CsN coated with polyethylene glycol (CsN-PEG), and (3) the DOXO coating with CsN-PEG to produce DOXO-CsN-PEG polymers. The prepared polymers were characterized using AFM, FE-SEM, and TEM analysis techniques. The cytotoxicity was evaluated on human blood cells. The redox reaction was studied using cyclic voltammetry (CV). The hemolysis percentage of blood treated with DOXO-CsN-PEG was less than 5% for all studied concentrations and no changing in red blood cells (RBCs) forms. The electrochemical study showed that the oxidation peak of blood was elevated when treated with DOXO, and decreased after treated with DOXO-CsN-PEG. The study concluded that the DOXO was converted from an oxidative agent to an antioxidant of blood when coated with CsN-PEG polymers.
