Analysis the Structure and Function of nsSNP of Interleukin 4 Gene by In-Silico Research
نویسندگان
1 College of Dentistry, Al-Bayan University, Baghdad, Iraq
2 Department of Medical Laboratory Technology, College of Health and Medical Techniques, AL-Bayan University, Baghdad, Iraq
3 Department of Medical Laboratory Techniques, University of Dijlah, Baghdad, Iraq
4 Department of Medical Microbiology, Faculty of Science and Health, Koya University, Koya KOY45, Kurdistan Region, Iraq
doi
10.22052/JNS.2025.01.012چکیده
Interleukin-4 is a type II inflammatory response cytokine. It is an essential component of the inflammatory response caused by an invasive allergen or parasite. Also, IL-4plays a key role in the up growth of inflammation and asthma by boost RI expression of the CF epsilon in B cells, mast cells and basophils, promoting the survival and proliferation of mast cells, and inducing chemotaxis of mast cells, basophils and eosinophils. We used six bioinformatics tools in this study (SIFT, pMut, PANTHER, Polyphen-2, PHDSNP, and SNPs&GO) prediction of disease-sensitive non-synonymous SNPs of IL4, IL-4’s seven nsSNPs (V53A, A118G, M144T, G2D, L110R, N113Y, and C123R) are predicted to be potentially harmful. The 3D structure of the abnormal protein was modelled by (HOPE), and the interaction between protein/protein was evaluated by STRING. While, Kaplan–Meier Plotter showed that the deregulation of IL4 expression affects the survival rate of ovarian cancer patients. Thus, IL4 may be significant as a gene marker for certain cancers. Study resolved harmful nsSNPs of IL-4, which could engage to the degradation of IL4 proteins and eventually lead to IL4 related diseases.
