فهرست نشریات علمی دسترسی آزاد ایران

The main purpose of this in vitro study was to evaluate the antibacterial activity of the selenium nanoparticles (Se-NPs) solution against gram-positive, and negative bacteria, and fungi. From ten samples of soil contaminated with petroleum products from various locations in Iraq, fifteen bacterial isolates were identified in the Ralstonia mannitolilytica from a soil sample. Ten isolates of Acinetobacter bumannii were isolated from burns and identified from hospitals in Iraq. Also, the minimum inhibitory concentration of (SeNps) against the growth of isolated A. bumannii was determined using the broth dilution method in a microtiter plate via serial dilution of (64, 32, 16, 8, and 4 μg/ml) of the antibacterial a wide of Gram-positive and Gram-negative bacteria was used to investigate the inhibitory activity and antifungal capabilities as Candida spp of the SeNPs. Color changes and UV-Vis spectroscopy were applied to confirm the initial synthesis of SeNPs and to explain the results. The presence of spherical SeNPs was demonstrated by energy-dispersive X-ray (EDX) analysis in conjunction with field-emission scanning electron microscopy (FESEM) on the surface of the bacterial biomass and in the supernatant solution. For molecular procedure, the MexA gene was detected in 10 isolates of MDR A. bumannii using real-time polymerase chain reaction (RT-PCR). The effect of SeNps on MexA gene expression revealed fold changes in expression (downregulation) in five isolates of MDR A. bumannii and upregulation in only one isolate of. The cytotoxicity of SeNPs by MTT assay showed that nanoparticles caused a decrease in viability of the tumor cell line (OECM-1) in a dose-dependent manner with a maximum inhibition of 24.5%.